Highlights

• Glyphosate exposure greatly impaired tomato's growth and development.

• Increased doses of glyphosate led to oxidative stress, especially in roots.

• Proline, ascorbate and glutathione levels were changed upon glyphosate exposure.

• Glyphosate stimulated the response of the enzymatic AOX system in a dose-dependent manner.

• Soil contamination by glyphosate can pose a serious threat to non-target plants.